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目的:从雌激素/雌激素相关受体信号通路探讨壮骨止痛方抗绝经后骨质疏松症的作用机制。方法:将60只雌性SD大鼠建立去卵巢骨质疏松症病理模型,将其随机分为壮骨止痛方高、中、低剂量组,模型组,雌二醇组,每组12只;另将12只雌性SD大鼠只切除卵巢附近等体积的脂肪组织作为假手术组。各组分别采用相对应药物进行灌胃治疗,治疗13周后各组随机选取6只雌鼠提取股骨组织mRNA样本进行PCR反应检测ERRα基因的表达,选取8只雌鼠经腹主动脉采血,凝固后离心分离出血清,应用雌二醇(E2)ELISA试剂盒检测血清E2水平。结果:与假手术组比较,模型组E2浓度及骨组织ERRα基因表达均显著降低,差异有统计学意义(P<0.01);与模型组比较,壮骨止痛方高、中剂量组及阳性对照组血清E2浓度及骨组织ERRα基因表达均显著升高,差异有统计学意义(P<0.01,P<0.05),壮骨止痛方低剂量组较模型组升高,但差异无统计学意义(P>0.05)。结论:壮骨止痛方通过同时提高去势雌鼠血清E2水平和骨组织ERRαmRNA表达来发挥抗绝经后骨质疏松症的作用。
OBJECTIVE: To explore the mechanism of Zhuangguzhitongfang on anti-postmenopausal osteoporosis from the estrogen / estrogen-related receptor signaling pathway. Methods: 60 female SD rats were established ovariectomized osteoporosis pathological model, which were randomly divided into high, medium and low dose Zhuanggu Zhitong Fang, model group, estradiol group, each group of 12; Twelve female SD rats were sacrificed by resection of equal volume of adipose tissue around the ovary. Each group were treated with the corresponding drugs for gavage. After 13 weeks of treatment, 6 females in each group were randomly selected to collect mRNA samples of femur tissues for detection of ERRα gene expression by PCR. Blood samples of 8 females were collected via abdominal aorta, The serum was separated by centrifugation and the level of serum E2 was detected by estradiol (E2) ELISA kit. Results: Compared with the sham operation group, the E2 level in the model group and the expression of ERRα gene in the bone tissue were significantly decreased (P <0.01). Compared with the model group, the high and medium dose Zhuanggu Zhitong Fang group and the positive control The concentration of serum E2 and the expression of ERRα gene in bone tissue were significantly increased, the difference was statistically significant (P <0.01, P <0.05). The low dose Zhuanggu Zhitong Fang group was higher than the model group, but the difference was not statistically significant P> 0.05). Conclusion: Zhuanggu Zhitong Prescription can exert anti-postmenopausal osteoporosis by increasing the level of serum E2 and the expression of ERRαmRNA in the bone tissue in ovariectomized female rats.