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目的:观察肾气丸对2型糖尿病阳虚证大鼠骨骼肌线粒体呼吸链复合体及ATP酶活性的影响。方法:将60只SD大鼠随机分为正常组12只和造模组48只,造模组48只大鼠予以高糖高脂饲料喂养3周后,采用小剂量链脲佐菌素加氢化可的松注射造模,观察2周制备2型糖尿病(阳虚证)大鼠,随机分为模型组、肾气丸组、补阳组、罗格列酮组。给药3周后处死大鼠,采用酶联免疫分析(ELISA)法分别测定大鼠骨骼肌线粒体呼吸链复合体及ATP酶的活性。结果:模型组Na~+-K~+-ATP酶、Ca~(2+)-Mg~(2+)-ATP酶以及线粒体呼吸链复合体Ⅰ、Ⅱ、Ⅲ、Ⅳ的活性低于正常组(P<0.01);肾气丸组、罗格列酮组Na~+-K~+-ATP酶、Ca~(2+)-Mg~(2+)-ATP酶以及线粒体呼吸链复合体Ⅰ、Ⅱ、Ⅲ、Ⅳ的活性明显高于模型组(P<0.05),但组间差异无统计学意义(P>0.05);补阳组Na~+-K~+-ATP酶、Ca~(2+)-Mg~(2+)-ATP酶以及线粒体呼吸链复合体Ⅰ、Ⅱ、Ⅲ、Ⅳ的活性明显低于肾气丸组(P<0.01)。结论:肾气丸能改善2型糖尿病阳虚证大鼠骨骼肌线粒体呼吸链复合体Ⅰ、Ⅱ、Ⅲ、Ⅳ及ATP酶的活性。
Objective: To observe the effect of Shenqi Pills on mitochondrial respiratory chain complex and ATPase activity in skeletal muscle of type 2 diabetic yang deficiency rats. METHODS: Sixty SD rats were randomly divided into 12 normal rats and 48 model rats. Forty-eight rats in the modeling group were fed with a high-sugar and high-fat diet for 3 weeks, and were then treated with low-dose streptozotocin. Cortisone injection was used to establish the model. Observe the type 2 diabetes (Yang Deficiency) rats in 2 weeks and randomly divide them into model group, Shenqi Pill group, Bu Yang group and Rosiglitazone group. After 3 weeks of administration, the rats were killed and the activity of mitochondrial respiratory chain complexes and ATPase in skeletal muscles of rats was determined by enzyme-linked immunosorbent assay (ELISA). Results: The activities of Na~+-K~+-ATPase, Ca~(2+)-Mg~(2+)-ATPase and mitochondrial respiratory chain complexes I, II, III and IV were lower in the model group than in the normal group. (P<0.01); Na~+-K~+-ATPase, Ca~(2+)-Mg~(2+)-ATPase and mitochondrial respiratory chain complex I in Shenqi Pills and rosiglitazone group The activity of II, III, IV was significantly higher than that of the model group (P<0.05), but there was no significant difference between the groups (P>0.05); Na~+-K~+-ATPase, Ca~ The activities of 2+)-Mg 2+ ATPase and mitochondrial respiratory chain complexes I, II, III and IV were significantly lower than those of Shenqi Pill group (P<0.01). Conclusion: Shenqi Pill can improve the activities of mitochondrial respiratory chain complex I, II, III, IV and ATPase in skeletal muscle of type 2 diabetes yang deficiency rats.