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目的:观察大鼠局灶性脑缺血再灌注后缺血脑区细胞间黏附分子-1(ICAM-1)以及P-选择素(P-selectin)的表达和黄连素对两者的影响。方法:将36只大鼠随机分为3组:假手术组,模型组,给药组。给药组分别于栓塞前15 min和栓塞后2 h,再灌注时ip给黄连素30 mg.kg-1;模型组和假手术组ip等量生理盐水。采用大脑中动脉线栓法制作大脑局灶缺血再灌注模型。再灌注24 h后取脑,应用免疫组化方法检测ICAM-1和P-selectin的表达。结果:黄连素能够显著降低缺血再灌注引起的脑梗死体积以及脑组织ICAM-1,P-selectin的表达增高(P<0.05)。其中梗死体积从25%降至17%;ICAM-1阳性细胞百分率从平均65%降至35%;P-selectin从平均60%降至30%。结论:黄连素能够通过脑缺血再灌注炎性反应的免疫调节机制保护脑缺血再灌注引起的损害。
Objective: To observe the expression of intercellular adhesion molecule-1 (ICAM-1) and P-selectin in the ischemic brain region and the effect of berberine on them after focal cerebral ischemia and reperfusion in rats. Methods: 36 rats were randomly divided into 3 groups: sham operation group, model group, and administration group. In the administration group, ip was given berberine 30 mg.kg-1 15 min before embolization and 2 h after embolization, and ip was equal to normal saline in the model group and sham operation group. A cerebral ischemic reperfusion model was established using the middle cerebral artery occlusion method. Brains were harvested 24 h after reperfusion, and the expression of ICAM-1 and P-selectin was detected by immunohistochemistry. RESULTS: Berberine could significantly reduce the volume of cerebral infarction caused by ischemia-reperfusion and the expression of ICAM-1 and P-selectin in brain tissue (P<0.05). The infarct volume decreased from 25% to 17%; the percentage of ICAM-1 positive cells decreased from an average of 65% to 35%; P-selectin decreased from an average of 60% to 30%. CONCLUSION: Berberine can protect the brain from the damage caused by cerebral ischemia-reperfusion through the immunoregulatory mechanism of cerebral ischemia-reperfusion inflammatory response.