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患儿,女,8岁。因发现阴道、尿道口有黄色粘液状分泌物,且有明显尿急、尿频、尿痛等尿路刺激征而就诊。在外院使用青霉素静滴、外用妇洁舒治疗1周无效。细菌学检查:直接涂片革兰氏染色见大量脓细胞50～100个/野(10×100),胞外找到G~-短粗杆菌外绕肥厚的荚膜,且多成对或短链状排列,细菌培养分离出肺炎克雷伯氏菌肺炎亚种。细菌鉴定:用灭菌生理盐水棉签采患儿阴道、尿道口分泌物(先用灭菌生理盐水清洗外阴),接种血平板,35℃,培养24h,菌落大,直径3～4mm,圆形,淡黄色,粘液状,用接种环沾取呈长丝
Children, female, 8 years old. Because of the vagina, urethra yellow mucus secretions, and urinary urgency, frequent urination, dysuria and other urinary tract irritation and treatment. Penicillin intravenous infusion in the outer courtyard, external Shu Jie Shu treatment for 1 week is invalid. Bacteriological examination: Direct smear Gram stain See a large number of pus 50 ~ 100 / wild (10 × 100), found extracellular G ~ - R. crude bacilli around the hypertrophy capsule, and multiple pairs or short chain Like arrangement, bacterial culture isolated Klebsiella pneumonia pneumonia subsp. Bacterial identification: Sterile saline cotton swab was used to collect children with vaginal and urethral discharge (first with sterile saline cleaning vulva), inoculation of blood plate, 35 ℃, culture 24h, colonies, diameter 3 ~ 4mm, round, Light yellow, mucus-like, inoculated with a loop was taken filaments