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目的探讨光动力疗法(PDT)抑瘤效应的免疫机制,以及PDT联合肿瘤局部注射卡介苗(BCG)的治疗效果。方法建立小鼠Heps肝癌移植瘤模型72只,随机分为对照组、PDT组、BCG组和联合组,每组18只。观测各组移植瘤体积的变化。采用酶联免疫吸附实验(ELISA),测定各组PDT治疗后2、24 h和7 d荷瘤小鼠血清中肿瘤坏死因子(TNF)-α的变化。结果 1.各治疗组治疗后7 d的移植瘤体积较对照组均明显缩小(P<0.01)。治疗后7 d联合组的肿瘤体积较PDT组明显缩小(P<0.05)。2.ELISA结果显示,治疗后2、24 h和7 dPDT组和联合组荷瘤小鼠血清中TNF-α的含量均明显高于对照组和BCG组(P<0.01),联合组荷瘤小鼠血清中TNF-α的含量明显高于PDT组(P<0.05)。结论 PDT可增强荷瘤小鼠全身的免疫力,肝癌移植瘤体积明显缩小。PDT联合局部注射BCG的疗效优于单用PDT和单用BCG。
Objective To investigate the immunological mechanism of photodynamic therapy (PDT) and the therapeutic effect of local injection of PDT combined with BCG. Methods Totally 72 Heps hepatocellular carcinoma xenografts were established and randomly divided into control group, PDT group, BCG group and combination group, with 18 rats in each group. The changes of tumor volume in each group were observed. The changes of serum tumor necrosis factor-α (TNF-α) in tumor-bearing mice at 2, 24 h and 7 d after PDT were determined by enzyme-linked immunosorbent assay (ELISA). Results 1. The volume of tumor xenografts in 7 days after treatment in each treatment group was significantly reduced compared with the control group (P <0.01). At 7 days after treatment, the tumor volume in the combined group was significantly reduced compared with PDT group (P <0.05). The results of ELISA showed that the levels of TNF-α in the serum of tumor-bearing mice at 2, 24 h and 7 d after treatment were significantly higher than those in the control and BCG groups (P <0.01) The content of TNF-α in rat serum was significantly higher than that in PDT group (P <0.05). Conclusion PDT can enhance the immunity of tumor-bearing mice, and the volume of hepatocellular carcinoma is significantly reduced. PDT combined with local injection of BCG is superior to PDT alone and BCG alone.