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作者报告用维多利亚苏木紫伊红双重染色法(VBHE)对血管内膜弹性纤维进行染色,来检测胃癌对血管的浸润,但对缺少弹性纤维的肿瘤浸润血管和粘膜毛细血管则难以有效.为此再用过氧化物酶-抗过氧化物酶的复合物(PAP)伴有因子Ⅷ-相关抗原和维多利亚蓝(FA Ⅷ:Ag VB)双重染色法来实
The authors reported the staining of intimal elastic fibers with Victorian hematoxylin red staining (VBHE) to detect the infiltration of blood vessels in gastric cancer, but it is difficult to effectively infiltrate blood vessels and mucosal capillaries in tumors lacking elastic fibers. This was further confirmed by the dual staining of the peroxidase-anti-peroxidase complex (PAP) with Factor VIII-associated antigen and Victoria Blue (FA VIII:Ag VB).