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目的:探讨全氟辛烷磺酸(PFOS)对大鼠的肾脏毒性。方法:清洁级SD大鼠24只,随机等分为4组,分别用含PFOS 0mg/kg、5mg/kg、25 mg/kg、125mg/kgPFOS的饲料喂养60d后,摘取眼球收集血液分离血清,检测血清中UREA、CRE、UA的含量;分离肾脏,测定肾脏器系数;然后一侧肾作常规病理学观察,另一侧肾组织匀浆分离上清液,测定MDA、GSH含量及GSH-Px、SOD活力。结果:与对照组比较,PFOS中、高剂量组肾脏器系数增加、血清UREA、CRE、UA含量升高(P<0.05);中、高剂量组肾组织出现明显的管型和蛋白物质渗出;低、中、高剂量组肾组织中MDA、GSH含量升高(P<0.05),GSH-Px、SOD活力降低(P<0.05),呈剂量-效应关系。结论:PFOS可对大鼠的肾脏产生损伤。
Objective: To investigate the renal toxicity of perfluorooctane sulfonate (PFOS) in rats. Methods: Twenty-four SD rats were randomly divided into four groups. After being fed with PFOS containing 0, 5, 25 and 125 mg / kg PFOS for 60 days respectively, the eyeballs were harvested for blood collection , The contents of UREA, CRE and UA in serum were detected; the kidneys were separated and the renal coefficient was measured; then the kidneys were observed by routine pathology, the supernatant was separated by the other kidney homogenate, the content of MDA and GSH, Px, SOD activity. Results: Compared with the control group, the renal organ coefficient increased and the levels of serum UREA, CRE and UA increased (P <0.05) in middle and high dose of PFOS group, and obvious tubular and protein exudation appeared in middle and high dose group (P <0.05). The activities of GSH-Px and SOD were decreased (P <0.05) in a dose-dependent manner in the low, medium and high dose groups. Conclusion: PFOS can damage the kidneys of rats.