论文部分内容阅读
目的:探讨蓝玉簪颗粒对脂多糖(LPS)诱导大鼠肺泡巨噬细胞(AM)内TNFα-表达及可能作用机制。方法:分离纯化AM,应用ELISA法检测蓝玉簪颗粒对LPS诱导的大鼠AM培养上清中的TNFα-水平的影响,应用Western blot方法检测大鼠AM内TNFα-及pERK蛋白表达水平,同时应用ERK拮抗剂(PD98059)观察AM内TNFα-蛋白表达。结果:蓝玉簪颗粒可剂量依赖的降低由于LPS刺激导致的AM培养上清内TNFα-含量升高;蓝玉簪(100 mg/L)颗粒可显著降低由于LPS刺激导致的AM细胞内pERK及TNFα-蛋白表达升高;ERK特异性抑制剂(PD98059 30 mol/L)及蓝玉簪颗粒干预,蓝玉簪颗粒+PD98059干预后,我们发现与LPS刺激组相比,大鼠AM中TNFα-表达显著降低。结论:蓝玉簪颗粒可以抑制LPS诱导AM TNFα-表达,其作用的机制之一可能与蓝玉簪抑制细胞外信号转导通路有关。
Objective: To investigate the possible mechanism of Lan YuZan Granules on lipopolysaccharide (LPS) -induced TNFα expression in rat alveolar macrophages (AM). Methods: AM was isolated and purified. The effect of Lan Yu Zan Granules on the TNFα level in LPS-induced AM supernatant was detected by ELISA. The expression of TNFα and pERK protein in AM was detected by Western blot. Meanwhile, ERK antagonist (PD98059) to observe TNF alpha-protein expression in AM. Results: Lan YuZan granules could reduce dose-dependently the increase of TNFα-content in AM culture supernatant induced by LPS stimulation. The blue alzheimer (100 mg / L) particles could significantly reduce the expression of pERK and TNFα- Compared with LPS stimulation group, the expression of TNFα in rat AM significantly decreased after ERK-specific inhibitor (PD98059 30 mol / L) and LanYanZan particles intervention and LanYanZan particles + PD98059 intervention. CONCLUSION: Lan Yu Zan Granules can inhibit LPS-induced AM TNFα-expression, and one of the mechanisms may be related to the inhibition of extracellular signal transduction pathway by Lan Yu Zan.