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采集发情犬和未发情犬卵巢 ,回收大腔 (直径大于 2mm)、小腔 (直径 1～ 2mm)和不可见卵泡卵母细胞 ,进行体外受精 ,结果如下 :①发情组的单精受精率 (31 2 % ) ,原核形成率 (1 9 4% )高于未发情组。发情组的多精受精率 (1 2 6% )要低于未发情组。②直径 1～ 2mm、大于 2mm卵泡组单精受精率略高于不可见卵泡组。可见卵泡组的原核形成率高于不可见卵泡组。③发情犬卵泡直径大于 2mm组卵裂率高于发情犬和未发情犬不可见卵泡卵母细胞。发情犬和未发情犬不可见卵泡卵裂率相差不显著
The estrus and non-estrus ovaries were collected, the large cavities (diameter> 2mm), small cavities (1 ~ 2mm in diameter) and invisible follicular oocytes were harvested for in vitro fertilization. The results were as follows: ①Single fertilization rate 31 2%), prokaryotic formation rate (1 94%) higher than the non-estrus group. The estrus fertilization rate of multiple fertilization (126%) than the non-estrus group. ② The diameter of 1 ~ 2mm, more than 2mm single sperm fertilization group slightly higher than the invisible follicle group. Visible follicular group prokaryotic formation rate was higher than the invisible follicular group. ③ the estrus follicle diameter greater than 2mm group cleavage rate is higher than that of estrus and non-estrus invisible follicular oocytes. Estrus and non-estrus invisible follicular cleavage rate difference was not significant