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目的:观察黄芪和地黄共同促人脐静脉内皮细胞血管新生的作用,并初步探讨其作用机制。方法:分别考察黄芪和地黄的混合提取物浓度为18.75,37.5,75mg/L,采用MTT法检测内皮细胞的增殖状况,通过划痕实验和Transwell实验考察内皮细胞的迁移能力,小管形成实验观察新生血管的形成,免疫印迹测定VEGFR2、Akt、p38、FAK的总蛋白及其磷酸化的表达。结果:药物浓度18.75mg/L不能提高体外成血管能力;37.5mg/L能促进内皮细胞增殖及成血管,并能显著提高p-FAK和p-VEGFR-2的表达,但不能促进内皮细胞迁移;75mg/L能促进内皮细胞增殖、迁移及成血管,并能显著提高p-Akt、p-FAK和p-VEGFR-2的表达。结论:黄芪和地黄诱导内皮细胞增殖、迁移和血管形成,促进血管新生,其机制可能与VEGF-VEGFR-2通路有关。
Objective: To observe the effect of Astragalus and rehmannia on angiogenesis of human umbilical vein endothelial cells and to explore its mechanism. Methods: The concentrations of mixed extracts of Radix Astragali and Rehmannia glutinosa were respectively tested as 18.75, 37.5 and 75 mg / L. The proliferation of endothelial cells was detected by MTT assay. The migration of endothelial cells was evaluated by scratch test and Transwell assay. The formation of blood vessels, immunoblotting, determined the total protein and its phosphorylated expression of VEGFR2, Akt, p38, FAK. Results: The drug concentration of 18.75mg / L could not increase the ability of angiogenesis in vitro. 37.5mg / L could promote the proliferation and angiogenesis of endothelial cells and significantly increase the expressions of p-FAK and p-VEGFR-2 but not promote the migration of endothelial cells ; 75mg / L can promote endothelial cell proliferation, migration and angiogenesis, and can significantly improve the expression of p-Akt, p-FAK and p-VEGFR-2. Conclusion: Astragalus and rehmanniae can induce the proliferation, migration and angiogenesis of endothelial cells and promote angiogenesis, which may be related to the VEGF-VEGFR-2 pathway.