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用落体撞击法复制大鼠闭合性脑损伤模型 ,经免疫组化法观察脑挫伤后不同时间GFAP、VIM的相关改变 ,并对其阳性细胞面积进行图像分析及统计学处理 ,旨在得出脑挫伤后GFAP、VIM表达的动态变化规律 ,为推断脑损伤时间的研究提供理论参考。结果显示 :GFAP阳性细胞在伤后 12h开始表达 ,7d达高峰 ,阳性细胞面积较对照组有显著差异 (P <0 0 1) ;VIM阳性细胞在伤后 3d开始表达 ,5d达高峰 ,阳性细胞面积较 3d组有显著差异 (P <0 0 5 )。GFAP及VIM阳性细胞均为星形胶质细胞 ,在损伤部位上 ,GFAP较VIM更为广泛 ,且反应更强烈。提示可用GFAP、VIM的动态观察作为推断脑损伤时间的一项指标
The rat model of closed brain injury was established by falling body collision method. The changes of GFAP and VIM at different time points after brain contusion were observed by immunohistochemical staining. The positive cell area was analyzed by image analysis and statistical method, The dynamic changes of GFAP and VIM expression after contusion provide a theoretical reference for the study on the time of brain injury. The results showed that GFAP positive cells began to express at 12h and peaked at 7d, the positive cell area was significantly different from that of the control group (P <0.01); VIM positive cells started to express 3d on the 5th day after injury, The area was significantly different from the 3d group (P <0 05). GFAP and VIM-positive cells are astrocytes, GFAP is more extensive than the VIM in the injury site, and the reaction is more intense. Tips Available GFAP, VIM dynamic observation as an index to infer the time of brain injury