The moving boundary non-equilibrium segregation during recrystallization and the influence of pre-deformation in Fe-30%Ni Alloy at1000℃ was investigated by the PTA (particle tracking autoradiography) technique, optical and electron microscopy. The results indicated that intensity of boron segregation on moving boundary after different deformation is concerned with the pre-deformation degree and the movingspeed of the boundary. The TEM ( transmission electron microscopy) result showed that the dislocation density nearby the moving boundary increase obviously. The phenomena are discussed by the widening grain boundary mechanism.