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实验采用阻断一侧大脑中动脉（MCAO）造成局灶性脑缺血模型，运用免疫组织化学方法测定脑缺血1h及3h脑组织中的TGF－β1阳性染色状况。结果显示：①脑缺血1h后缺血测与缺血对侧、缺血侧与对照组的TGF－β1染色均有显著性差异（P＜0．05）；而缺血中心和缺血半影区的比较无计学意义（P＞0．05）；②脑缺血3h后的TGF－β1表达较缺血1h有新的变化，缺血半影区阳性率呈上升趋势而缺血中心则呈下降趋势（93．3％vs100％，73．3％vs60％），其变化具有显著性差异（P＜0．05）。表明脑缺血后神经细胞TGF－β1表达增高及TGF－β1可能参与神经细胞抗损伤机制。
The model of focal cerebral ischemia was established by blocking the middle cerebral artery (MCAO). The immunohistochemistry was used to detect the positive staining of TGF-β1 in the brain tissue 1h and 3h after cerebral ischemia. The results showed: (1) There was a significant difference in the expression of TGF-β1 between ischemia and contralateral ischemic and ischemic sides 1h after cerebral ischemia (P <0.05); while ischemic center and ischemic half (P> 0.05). ② The expression of TGF-β1 in ischemic brain tissue after 1h of ischemic insult had a new change compared with that of 1h after ischemic insult, the positive rate of ischemic penumbra increased and the ischemic center (93.3% vs100%, 73.3% vs60%), the difference was significant (P <0.05). It indicated that the expression of TGF-β1 in nerve cells increased and the expression of TGF-β1 might be involved in the anti-injury mechanism of nerve cells after cerebral ischemia.