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目的:探讨B型钠尿肽(BNP)抑制心成纤维细胞(CFs)增殖机制与转化生长因子-β1(transforming growth factor-β1,TGF-β1)/Smad信号传导途径的关系。方法:原代提取乳鼠的心CFs,以重组人心肌营养素刺激细胞增殖,分为:BNP组(A组)、SB431542组(B组)、BNP+SB431542组(C组)、对照组(D组)。收集培养液检测细胞因子TGF-β1,收集细胞提取总RNA以荧光实时定量RT-PCR检测Smad4 mRNA变化。结果:A、B、C组TGF-β1浓度较D组明显降低而Smad4 mRNA的△CT值较D组明显升高(P<0.05),但A、B、C组之间TGF-β1相比差异无统计学意义(P>0.05);C组Smad4 mRNA△CT值高于A组和B组。结论:BNP抑制CFs增殖机制除了TGF-β1/Smad2/3信号途径,还可能存在TGF-β1非Smad2/3信号传导途径。
Objective: To investigate the relationship between the inhibitory effect of B-type natriuretic peptide (BNP) on the proliferation of cardiac fibroblasts (CFs) and the signal transduction pathway of transforming growth factor-β1 (TGF-β1) / Smad. Methods: Primary cardiac CFs were extracted from neonatal rat hearts and stimulated with recombinant human cardiomyocyte stimulator. The cells were divided into three groups: BNP group (group A), SB431542 group (group B), BNP + SB431542 group (group C) group). The culture medium was collected to detect the expression of TGF-β1. The total RNA was collected and the changes of Smad4 mRNA were detected by real-time quantitative RT-PCR. Results: The concentration of TGF-β1 in group A, B and C was significantly lower than that in group D and the △ CT value of Smad4 mRNA in group A, B and C was significantly higher than that in group D (P <0.05) The difference was not statistically significant (P> 0.05). The △ CT value of Smad4 mRNA in group C was higher than that in group A and group B. CONCLUSION: BNP can inhibit the proliferation of CFs in addition to TGF-β1 / Smad2 / 3 signaling pathway, there may be TGF-β1 non-Smad2 / 3 signaling pathway.