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目的:用基因工程的方法对乙型肝炎病毒核心抗原在大肠杆菌中的构建及表达进行分析与探讨。方法:利用ELISA方法,使得HBcAg基因片段扩增,构建含有HBcAg基因的表达质料与克隆质粒,以IPTG诱导大肠杆菌BL21(DE3)plys中蛋白的重组表达,并且,采用酶联免疫测定方法检测重组蛋白。结果:在大肠杆菌中,重组HBcAg得到表达,经过酶联免疫测定方法检测,在预期位置显示有特异性条带。结论:采用基因工程的方法对乙型肝炎病毒核心抗原在大肠杆菌中的构建及表达,成功构建了HBcAg原核表达系统,并获取重组HBcAg,有效地推进了重组蛋白的相关功能研究,具有很高的研究推广价值。
OBJECTIVE: To analyze and discuss the construction and expression of hepatitis B virus core antigen in Escherichia coli by genetic engineering. Methods: HBcAg gene fragment was amplified by ELISA, and the expression plasmid containing HBcAg gene and the cloned plasmid were constructed. The recombinant protein was induced by IPTG in E. coli BL21 (DE3) plys and the recombinant protein was detected by enzyme-linked immunosorbent assay protein. Results: In E. coli, recombinant HBcAg was expressed and detected by ELISA. Specific bands were shown at the expected sites. Conclusion: The HBcAg prokaryotic expression system was successfully constructed and the recombinant HBcAg was successfully constructed by using genetic engineering to construct and express hepatitis B virus core antigen in Escherichia coli, which effectively promoted the study of the related function of recombinant protein. The research promotion value.