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目的研究亚慢性染铝对断乳大鼠神经行为学和神经细胞形态学的影响,以明确铝对发育中的中枢神经系统的毒性作用,为机制研究提供理论依据和实验资料。方法对刚断乳Wistar大鼠通过饮水亚慢性连续染毒12周,采用原子吸收石墨炉法进行血铝和脑铝含量的测定;跳台试验法观察大鼠学习、记忆行为学的改变;HE染色和尼氏染色后,光镜观察海马形态学改变。结果高剂量染铝组脑组织系数显著低于对照组;随着染铝剂量的增加,染铝组血铝和脑铝浓度显著升高(P<0.05,P<0.01),低、高剂量组之间脑铝差异有统计学意义(P<0.05);大鼠跳台试验的潜伏期随铝暴露剂量增加逐渐缩短,而错误次数逐渐增加,有明显的剂量-效应关系(P<0.01)。海马HE和尼氏染色后光镜观察表明,各染铝组与对照组相比均未发现明显差异。结论亚慢性铝暴露可以引起发育中大鼠学习记忆能力下降,但其形态学观察未见器质性病变。
Objective To investigate the effects of subchronic aluminum on neurobehavioral and neuronal morphological changes in weaned rats to clarify the toxic effects of aluminum on developing central nervous system and to provide theoretical basis and experimental data for the mechanism study. Methods Wistar rats were sub-chronic continuous exposure to drinking water for 12 weeks. The contents of aluminum and aluminum were measured by atomic absorption graphite furnace method. The changes of learning and memory behavior were observed by jumping test. And Nissl staining, light microscopy changes in hippocampal morphology. Results Compared with the control group, the brain tissue index of high-dose aluminum-exposed group was significantly lower than that of the control group. With the increase of aluminum dose, the concentrations of aluminum, aluminum and aluminum in the aluminum-treated group were significantly increased (P <0.05, P <0.01) (P <0.05). The latency of platform jump test in rats gradually shortened with the increasing dose of aluminum exposure, and the number of errors gradually increased with a significant dose-response relationship (P <0.01). Histoplasmic HE and Nissl staining after light microscopy showed that the dye aluminum group compared with the control group were found no significant difference. Conclusion Subchronic aluminum exposure can cause the learning and memory abilities of developing rats to decline, but no morphological changes are observed in the morphological observation.