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目的研究Skp2和P27蛋白在食管鳞癌中表达,探讨它们之间的关系及其临床意义。方法应用免疫组化Sp法检测70例食管鳞癌中Skp2和P27蛋白的表达并分析相关性。结果 Skp2蛋白在癌旁正常组织、不典型增生及食管鳞癌组中阳性表达率分别为10．00%(2／20)、37．04%(10／27)、41．43% (29／70),后两者的阳性率显著高于前者(P<0．05)。P27在正常组织的阳性率为85．00％(17／20),高于食管鳞癌组48．57％(34／70),其差异有显著性(P<0．01)。Skp2蛋白在食管鳞癌组织中表达Ⅲ-Ⅳ期高于Ⅰ～Ⅱ期(P <0．05),且与淋巴结转移显著正相关。P27蛋白表达与食管癌淋巴结转移负相关,Skp2蛋白表达与P27表达呈负相关。Skp2表达阳性及P27表达阴性提示预后较差(5年累积生存率40．63％,P<0．05)。结论 Skp2 和P27蛋白表达的检测可能成为判断食管鳞癌预后的指标,指导患者的临床治疗,并为早期诊断食管鳞癌提供参考。
Objective To study the expression of Skp2 and P27 in esophageal squamous cell carcinoma and to explore the relationship between them and their clinical significance. Methods The expression of Skp2 and P27 protein in 70 cases of esophageal squamous cell carcinoma was detected by immunohistochemical SP method and the correlation was analyzed. Results The positive rates of Skp2 protein in adjacent normal tissues, atypical hyperplasia and esophageal squamous cell carcinoma tissues were 10.00% (2/20), 37.04% (10/27), 41.43% (29 / 70), the positive rate of the latter two was significantly higher than the former (P <0.05). The positive rate of P27 in normal tissue was 85.00% (17/20), which was higher than that in esophageal squamous cell carcinoma (48.57%, 34/70) (P <0.01). Skp2 protein expression in esophageal squamous cell carcinoma stage Ⅲ-Ⅳ was higher than Ⅰ ~ Ⅱ (P <0.05), and lymph node metastasis was significantly correlated. P27 protein expression was negatively correlated with lymph node metastasis in esophageal cancer, Skp2 protein expression was negatively correlated with P27 expression. The positive expression of Skp2 and negative expression of P27 suggest poor prognosis (5-year cumulative survival rate 40.63%, P <0.05). Conclusion The detection of Skp2 and P27 protein expression may be an index to judge the prognosis of esophageal squamous cell carcinoma and guide the clinical treatment of esophageal squamous cell carcinoma and provide a reference for the early diagnosis of esophageal squamous cell carcinoma.