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本文介绍了以蛋白结合竞争法测定人外周血淋巴细胞中环磷酸腺苷的试验原理、测定和鉴定方法。(1)以全血低速离心和双层分层液方法提纯淋巴细胞,其纯度达90%左右;(2)以1 N高氯酸破坏淋巴细胞;(3)以蛋白结合竞争法测定环磷酸腺苷水平。我们测定了23名健康人外周血淋巴细胞环磷酸腺苷水平,结果为2.98±1.04微微克分子/10~6淋巴细胞。
This paper introduces the principle, determination and identification of cAMP in human peripheral blood lymphocytes by the protein binding competition method. (1) Purification of lymphocytes by whole blood low-speed centrifugation and double-layer stratified liquid method with the purity of about 90%; (2) Destruction of lymphocytes by 1 N perchloric acid; (3) Determination of cyclophosphamide by protein binding competition assay Adenosine levels. We measured cAMP levels in 23 healthy human peripheral blood lymphocytes and found 2.98 ± 1.04 pg / 10 ~ 6 lymphocytes.