论文部分内容阅读
目的：观察去甲二氢愈创木酸（ＮＤＧＡ）对人恶性胶质瘤细胞系ＳＨＧ－４４细胞增殖和分化的影响。方法：细胞培养、免疫组化、光镜和电镜观察及流式细胞仪检测。结果：①在２００μｍｏｌ／Ｌ浓度范围内，ＮＤＧＡ对ＳＨＧ－４４细胞有增殖抑制和诱导分化作用，且这种作用与浓度呈正相关；②在ＮＤＧＡ处理９６ｈ内，ＮＤＧＡ对ＳＨＧ－４４细胞的增殖抑制作用以处理后２４ｈ最明显；③ＮＤＧＡ处理后，ＳＨＧ－４４细胞增殖受阻于Ｇ１→Ｓ期。结论：ＮＤＧＡ对ＳＨＧ－４４细胞有明显的增殖抑制和诱导分化作用，且这种作用有周期特异性。
Objective: To observe the effects of nordihydroguaiaretic acid (NDGA) on the proliferation and differentiation of human glioblastoma cell line SHG-44. Methods: Cell culture, immunohistochemistry, light and electron microscopy and flow cytometry. RESULTS: ① In the concentration range of 200 μmol / L, NDGA had inhibitory and differentiation-inducing effect on SHG-44 cells, and this effect was positively correlated with the concentration. (2) NDGA inhibited the proliferation of SHG-44 cells 24h after treatment was the most obvious; ③NDGA treatment, SHG-44 cell proliferation blocked in the G1 → S period. CONCLUSION: NDGA can significantly inhibit SHG-44 cells proliferation and induce differentiation, and this effect has cycle specificity.