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目的:探讨凉血散瘀汤治疗实验性脑出血大鼠的可能机制。方法:将60只Wistar大鼠以随机表法随机均分6组:空白组、假手术组、模型组、治疗1组,治疗2组、治疗3组。后4组冬采用2次注射自体动脉血法构建大鼠脑出血模型,术后3h治疗1、2和3组分别给予凉血散瘀汤2.4、4.8和7.2mg/(kg.d)灌胃,其他3组给予等量生理盐水。应用放射免疫法测定血ETand TNF-α含量。结果:与空白组比较,假手术组血ET和TNF-α轻度升高(P>0.05);与假手术组比较,模型组血ET和TNF-α明显升高(P<0.01);与模型组比较,治疗组血ET和TNF-α明显下降(P<0.01);治疗组间比较血ET和TNF-α下降差不多。模型组和治疗组FIB水平较正常组升高(P<0.01)。模型组和治疗组TT与正常组比较并无明显差异(P>0.05)。结论:凉血散瘀汤治疗大鼠脑出血后对神经功能具有保护作用,其机制可能抑制ET表达、TNF-α毒性和降低FIB活性有关。
Objective: To investigate the possible mechanism of Liangxue Sanyu Decoction in treating experimental cerebral hemorrhage rats. Methods: Sixty Wistar rats were randomly divided into 6 groups randomly: blank group, sham group, model group, treatment group 1, treatment group 2 and treatment group 3. After the second four groups of rats, autologous arterial blood injection was used to establish an intracerebral hemorrhage model in rats. After treatment, groups 1, 2 and 3 were given intragastric administration of Liangxue Sanyu Decoction 2.4, 4.8 and 7.2 mg/(kg.d) respectively. The other three groups were given the same amount of saline. Radioimmunoassay was used to determine the content of ETand TNF-α in blood. Results: Compared with the blank group, ET and TNF-α levels in the sham operation group increased slightly (P>0.05). Compared with the sham group, the ET and TNF-α levels in the model group increased significantly (P<0.01). Compared with the model group, the blood ET and TNF-α in the treatment group decreased significantly (P<0.01); the blood ET and TNF-α decreased in the treatment group. The levels of FIB in the model group and the treatment group were higher than those in the normal group (P<0.01). There was no significant difference in TT between the model group and the treatment group compared with the normal group (P>0.05). Conclusion: Liangxue Sanyu Decoction has a protective effect on neurological function after cerebral hemorrhage in rats. The mechanism may be related to inhibiting ET expression, TNF-α toxicity and reducing FIB activity.