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目的 研究临床应用浓度的三氧化二砷 (As2 O3 )是否具诱变致癌性。方法 应用姐妹染色单体分化染色技术 ,检测不同浓度的As2 O3 对正常人外周血淋巴细胞姐妹染色单体的影响。结果 As2 O3 的浓度≥ 10 -6mol/L时 ,淋巴细胞姐妹染色单体互换 (SCE)频率与对照组相比显著升高 (P <0 0 1) ,细胞生长停滞于M1期 ,细胞分裂明显减少 ,并呈剂量效应关系 ;As2 O3 的浓度≤ 10 -7mol/L时 ,淋巴细胞的SCE频率、细胞周期和细胞分裂指数与对照组相比差异无显著性 (P >0 .0 5 )。结论 As2 O3 在临床应用时有以下两个潜在风险 :①可能诱发第 2次肿瘤 ;②可能影响淋巴细胞的免疫功能。
Objective To investigate whether clinically applied concentrations of arsenic trioxide (As2O3) are mutagenic and carcinogenic. Methods Sister chromatid differentiation staining technique was used to detect the effect of different concentrations of As2O3 on sister chromatid of peripheral blood lymphocytes in normal subjects. Results When the concentration of As2O3 ≥ 10 -6 mol/L, the frequency of sister chromatid exchange (SCE) of lymphocytes was significantly higher than that of the control group (P < 0 01), cell growth arrested in the M1 phase, cell division. Significantly decreased and showed a dose-effect relationship; when the concentration of As2O3 was ≤10 -7 mol/L, the SCE frequency, cell cycle, and cell division index of lymphocytes were not significantly different from those in the control group (P > 0.05). . Conclusion There are two potential risks for As2O3 in clinical application: 1 It may induce the second tumor; 2 It may affect the immune function of lymphocytes.