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The meso-tetra(α,α,α,α-O-phenylacetylbenzene)porphyrin was used as a complete antigen to elicit monoclonal antibody 1F2 through the immunization and cell fusion techniques. The antibody could combine with Mn porphyrin and showed a haloperoxidase activity. The affinities of the antibody for antigen and Mn porphyrin were 4 98×10 -5 mol/L and 7 63×10 -5 mol/L, respectively. When the antibody-Mn porphyrin complex was used to catalyze the halogenation reaction, the Michaelis constant and reactive rate constant κ cat for H 2O 2 were 5 127 0×10 -3 mol/L and 0 483 4 s -1, respectively. The enzyme exchange number κ cat/K m=94 285 1 mol/(L·s).
The meso-tetra (α, α, α-O-phenylacetylbenzene) porphyrin was used as a complete antigen to elicit monoclonal antibody 1F2 through the immunization and cell fusion techniques. The antibody could combine with Mn porphyrin and showed haloperidone activity. The affinities of the antibody for antigen and Mn porphyrin were 4 98 × 10 -5 mol / L and 7 63 × 10 -5 mol / L, respectively. When the antibody-Mn porphyrin complex was used to catalyze the halogenation reaction, the Michaelis constant and reactive rate constant κ cat for H 2 O 2 were 5 127 0 × 10 -3 mol / L and 0 483 4 s -1, respectively. The enzyme exchange number κ cat / K m = 94 285 1 mol / (L · s).