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我们建立了ｂ 型流感嗜血杆菌（Ｈｉｂ）外膜蛋白（ＯＭＰ）特异抗体测定的间接酶联免疫吸附法（ＥＬＩＳＡ），同时联合血、尿细菌抗原检测，对７０ 例肺炎患儿及３０ 例健康儿童进行研究。结果有１９／７０（２７．１％ 例）肺炎患儿产生Ｈｉｂ ＯＭＰ抗体反应，除外１ 例交叉反应，余１８ 例诊断为Ｈｉｂ 感染，其中５ 例未查到Ｈｉｂ 抗原，以Ｈｉｂ ＯＭＰ特异抗体反应得到诊断。表明Ｈｉｂ ＯＭＰ抗体测定可提高小儿Ｈｉｂ 肺炎的病原诊断率。
We established an indirect enzyme-linked immunosorbent assay (ELISA) for the determination of Hib anti-OMP antibodies and combined detection of blood and urine bacterial antigens. Seventy patients with pneumonia and 30 Healthy children study. RESULTS: Hib OMP antibody was detected in 19/70 (27.1%) children with pneumonia, except for one case of cross-reaction, and the remaining 18 cases were diagnosed as Hib infection. No Hib antigen was detected in 5 cases and Hib OMP specific antibody Get diagnosed. Hib OMP antibody test can increase the diagnosis of pediatric Hib pneumonia pathogens.