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【目的】探明OsCYP2基因导入草莓过表达对其植株耐盐性的影响。【方法】利用农杆菌介导的叶盘转化法将耐盐OsCYP2基因转入到草莓基因组中,探讨农杆菌菌液浓度、侵染时间等影响草莓转化效率的主要因子及NaCl胁迫处理对转基因植株最大光化学效率及叶绿素含量的影响。【结果】筛选出菌液浓度OD6000.8、侵染时间5 min为适宜的转化条件,得到了较理想的转化效果,经PCR检测证实OsCYP2基因已整合到草莓基因组中,并在草莓植株中超量表达。在高盐条件下,转基因植株PSⅡ最大光化学效率(Fv/Fm)及叶绿素a、叶绿素b和类胡萝卜素含量明显高于未转化对照植株。【结论】说明在盐胁迫下OsCYP2基因不仅可以调控草莓叶片光合色素合成,而且可以保护草莓植株叶片光系统II的光化学效率,维持正常的光合作用,从而提高植株的耐盐性。
【Objective】 The objective of this study was to investigate the effect of overexpression of OsCYP2 gene on the salt tolerance of plants. 【Method】 The salt-tolerant OsCYP2 gene was introduced into the genome of strawberry by Agrobacterium-mediated leaf disc transformation. The main factors affecting the transformation efficiency of Agrobacterium tumefaciens, such as Agrobacterium tumefaciens concentration and infection time, and the effects of NaCl stress on transgenic plants Maximum photochemical efficiency and chlorophyll content. 【Result】 The optimal transformation conditions were obtained when the concentration of OD6000.8 and the infection time of 5 min were selected. The results showed that the OsCYP2 gene had been integrated into the genome of strawberry and had been over-expressed in strawberry plants expression. Under high salt stress, the maximum photochemical efficiency of PSⅡ (Fv / Fm), chlorophyll a, chlorophyll b and carotenoid content of transgenic plants were significantly higher than those of untransformed control plants. 【Conclusion】 OsCYP2 gene can regulate the photosynthetic pigment synthesis of strawberry leaves under salt stress and protect the photosynthetic efficiency of strawberry plant photosystem II and maintain the normal photosynthesis, thus improving the salt tolerance of the plants.