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目的:研究复方丹酚滴丸中不同质量浓度冰片配伍丹酚酸后对丹酚酸B,紫草酸,迷迭香酸3个成分在小肠的吸收速率常数(Ka)和表观吸收系数(P_(app))的影响。方法:采用大鼠在体单向肠灌注试验考察吸收过程,以HPLC测定丹酚酸B,紫草酸和迷迭香酸的含量,流动相1%冰乙酸水溶液(A)~(~(-1))%冰乙酸甲醇溶液(B)梯度洗脱(0~10 min,10%~20%B;10~40min,20%~47%B;40~50 min,47%~70%B),检测波长286 nm,流速0.8 m L·min~(~(-1))。结果:高、中、低质量浓度冰片配伍丹酚酸后,Ka和Papp大多有所增加,其中以100 mg·L~(~(-1))冰片组的促吸收效果最优,该组丹酚酸B,紫草酸,迷迭香酸的全肠段Ka分别为不加冰片的2.31,2.33,3.38倍。结论:不同浓度冰片配伍丹酚酸后,对丹酚酸B,紫草酸和迷迭香酸在大鼠各肠段的吸收具有一定促进作用,浓度对促吸收效果有一定影响;增加冰片的浓度,促吸收作用有所提高,但当冰片到一定浓度后,促吸收作用有所减弱。
OBJECTIVE: To study the absorption rate constants (Ka) and apparent absorption coefficients (Ps) of three components of salvianolic acid B, lithospermate and rosmarinic acid in Compound Dan-Dan Dropping Pills with different concentrations of borneol combined with salvianolic acid (app)). Methods: The rat intestinal absorption test was carried out in vivo. The contents of salvianolic acid B, lithospermate and rosmarinic acid were determined by HPLC. The mobile phase consisted of 1% glacial acetic acid (A) ~ (-1 ))% Glacial acetic acid in methanol (B) gradient (0-10 min, 10-20% B; 10-40 min, 20-47% B; 40-50 min, 47-70% B) The detection wavelength was 286 nm and the flow rate was 0.8 m L · min ~ (-1). Results: Ka, Papp mostly increased with high, medium and low concentration of borneol combined with salvianolic acid, of which 100 mg · L ~ (-1) borneol had the best absorption promoting effect, Phenolic acids B, lithospermic acid, rosmarinic acid, the whole bowel segment Ka, respectively, without Borneol 2.31,2.33,3.38 times. CONCLUSION: The combination of salvianolic acid with different concentrations of borneol can promote the absorption of salvianolic acid B, lithospermate and rosmarinic acid in the intestine of rats, and the concentration of salvianolic acid has a certain influence on the absorption promoting effect. Increasing the concentration of borneol , Promote the absorption effect has increased, but when the ice to a certain concentration, the role of promoting absorption weakened.