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分析慢性移植肾肾病(CAN)患者与移植物功能稳定肾移植受者外周血基因表达差异,探索CAN免疫致病机制。从GEO数据库获取GSE12187和GSE22229两个肾移植受者外周血的基因芯片表达谱数据集,选取其中13个CAN样本为实验组,15个移植物功能稳定的样本为对照组。以SAM筛选两组样本的差异表达基因。应用DAVID及GENECODIS网络工具对差异基因进行功能富集分析。通过Cytoscape软件的MiMI插件进行差异基因的蛋白质-蛋白质相互作用(PPI)网络分析。SAM筛选出上调基因168个,下调基因141个。基因富集结果显示,上调基因主要为调控转录和翻译过程的基因,而下调基因参与广泛信号传导通路以及基因表达的调控。Cytoscape软件的PPI分析筛选出19个CAN相关核心基因。CAN患者与移植肾功能稳定受者的免疫功能状态存在显著差异;CAN致病机制涉及免疫细胞复杂的基因表达调控及细胞增值、凋亡、迁移等广泛的信号传导通路变化;免疫细胞在基因的转录、RNA加工、翻译及蛋白质代谢等环节的改变可能在CAN的致病中发挥了关键作用;TAGAP、EIF3F、NUDT21、PAPOLA、RPL等CAN核心基因的免疫调控机制需要深入探索。
To analyze the differences of gene expression in peripheral blood of patients with chronic allograft nephropathy (CAN) and those with stable graft function and to explore the mechanism of CAN immune pathogenesis. The gene chip profiling datasets of peripheral blood from two kidney transplant recipients GSE12187 and GSE22229 were obtained from the GEO database. Thirteen CAN samples were selected as the experimental group and the 15 grafts with stable function as the control group. SAM differentially expressed genes were screened for two groups of samples. Using DAVID and GENECODIS network tools to enrich and analyze the differential genes. Differential gene protein-protein interaction (PPI) network analysis was performed using the MiMI plugin for Cytoscape software. There were 168 up-regulated genes and 141 down-regulated genes in SAM. Gene enrichment results show that the up-regulated genes are mainly genes that regulate transcription and translation processes, while the down-regulated genes are involved in a wide range of signal transduction pathways and regulation of gene expression. Cytoscape software PPI analysis screened 19 CAN related core genes. The immune function status of CAN patients and recipients with stable renal transplant recipients is significantly different. The pathogenesis of CAN involves the complex regulation of immune cells and the extensive signal transduction pathways such as cell proliferation, apoptosis and migration. The immune cells in the gene The changes of transcription, RNA processing, translation and protein metabolism may play a key role in the pathogenesis of CAN. The mechanisms of immunoregulation of CAN core genes such as TAGAP, EIF3F, NUDT21, PAPOLA and RPL need further exploration.