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目的探讨肿瘤坏死因子-β(TNF-β)基因型多态性与膀胱肿瘤的关系。方法用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法,检测所有研究对象的TNF-β基因+A252G位点的基因型,探讨其基因型与膀胱肿瘤的关系。结果TNF-β基因型多态性与膀胱肿瘤的发病率相关,且膀胱肿瘤患者中,TNF-β基因型多态性与肿瘤的分级相关(P<0.01)。结论检测膀胱肿瘤患者的TNF-β基因型多态性有助于为研究膀胱肿瘤的发生发展提供一个新的思路。
Objective To investigate the relationship between tumor necrosis factor-β (TNF-β) genotype and bladder tumor. Methods The genotypes of TNF-β + A252G in all the subjects were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the relationship between genotype and bladder tumor was explored. Results The genotype of TNF-β was correlated with the incidence of bladder cancer. Among the patients with bladder cancer, the polymorphism of TNF-β was associated with tumor grade (P <0.01). Conclusion Detection of TNF-β genotype polymorphism in bladder cancer patients is helpful for the study of the occurrence and development of bladder cancer.