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目的建立4300紫外可见分光光度计检测血中碳氧血红蛋白含量方法快速诊断一氧化碳(CO)中毒。方法检血用0.1%Na2CO3稀释,加入连二亚硫酸钠,4300紫外可见分光光度计测定500nm~600nm吸收光谱和538nm和555nm处吸光度值,确定碳氧血红蛋白,计算碳氧血红蛋白含量。结果氧和血红蛋白的最大吸收波长为555nm,碳氧血红蛋白的最大吸收波长为538nm和555nm。4300紫外可见分光光度计检测血中碳氧血红蛋白含量(10%~90%)回收率平均为95%~110%,标准差为2.1%~8.0%,最低检出浓度为2%。80例可疑CO中毒者中63例血中检出碳氧血红蛋白,平均含量为35%。168例可疑CO中毒死亡者中124例血中检出碳氧血红蛋白。结论4300紫外可见分光光度计检测血中碳氧血红蛋白,具有快速、准确、操作简便、结果可靠的特点,可应用于CO中毒的诊断和CO中毒死亡的法医学鉴定。
Objective To establish a 4300 UV-Vis spectrophotometer to detect the content of carboxyhemoglobin in blood for rapid diagnosis of carbon monoxide (CO) poisoning. Methods Blood samples were diluted with 0.1% sodium bicarbonate and sodium dithionite was added. The absorbance at 500nm ~ 600nm and the absorbance at 538nm and 555nm were measured by 4300 UV - Vis spectrophotometer. The content of carboxyhemoglobin was calculated. Results The maximum absorption wavelength of oxygen and hemoglobin was 555 nm, and the maximum absorption wavelengths of carboxyhemoglobin were 538 nm and 555 nm. 4300 UV-Vis spectrophotometer blood carboxyhemoglobin content (10% to 90%) average recovery of 95% to 110%, standard deviation of 2.1% to 8.0%, the lowest detection rate of 2%. Among the 80 cases of suspected CO poisoning, carboxyhemoglobin was detected in 63 cases with the average content of 35%. Of the 168 patients with suspected CO poisoning, 124 were found to have carboxyhemoglobin. Conclusion 4300 UV-Vis spectrophotometer is a fast, accurate, simple and reliable method for the determination of carboxyhemoglobin in blood. It can be used in the diagnosis of CO poisoning and forensic identification of CO poisoning death.