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本文用HPLC法对中成药九分散中麻黄碱、伪麻黄碱及士的宁进行了分离及含量测定。与药典法比较,本法的提取率较高,其中麻黄碱为药典方法的1.33倍,伪麻黄碱为1.29倍,而士的宁则与药典方法相当。在μBondapak C_(18)色谱柱上,用MeOH—KH_2PO_4溶液为流动相梯度洗脱进行了各组分的分离。各组分工作曲线线性良好,麻黄碱,伪麻黄碱在0～8μg、士的宁0～14μg范围内呈线性关系。测得回收率为麻黄碱98.94±2.2%;伪麻黄碱97.37±1.9%;士的宁100.7±1.9%。
In this paper, ephedrine, pseudoephedrine and strychnine were separated and determined by HPLC method. Compared with the pharmacopoeia method, the extraction rate of this method is relatively high, with ephedrine being 1.33 times that of the pharmacopoeia method and pseudoephedrine being 1.29 times, while strychnine is comparable to the pharmacopoeia method. On the μBondapak C_(18) column, the separation of the components was carried out with a gradient of MeOH-KH_2PO_4 as the mobile phase. The working curves of the components were linear, and the ephedrine and pseudoephedrine were linear in the range of 0 to 8 μg and strychnine 0 to 14 μg. The recoveries were 98.94±2.2% for ephedrine, 97.37±1.9% for pseudoephedrine, and 100.7±1.9% for strychnine.