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本研究利用分布于水稻12条染色体上的12个SSR标记构建了太湖稻区10份抗褐飞虱粳稻的DNA指纹图谱,并对粳稻品种之间的遗传相似性进行了分析,旨在为水稻抗性育种、种子纯度鉴定和新品种知识产权保护方面提供支持。研究结果显示:12对引物共检测到35个等位基因,每对SSR引物检测到等位基因为1~6个,平均为2.8个;筛选出3对核心引物(RM336,RM5414,RM190)并建立了10份抗褐飞虱粳稻的SSR指纹图谱;10份粳稻品种之间的相似系数变幅为0.20~0.91,平均为0.63,遗传相似系数在0.50以上的材料占全部的82.22%。UPGMA聚类结果显示:在遗传相似系数0.55处,10份粳稻材料可以分为2个类群。研究结果表明,太湖地区绝大多数抗褐飞虱粳稻品种遗传相似性高,遗传多样性不够丰富。
In this study, 12 SSR markers distributed on 12 chromosomes of rice were used to construct the DNA fingerprint of 10 brown planthopper (Oryza sativa L.) japonica cultivars in the rice plantation of Taihu Lake. The genetic similarity among japonica rice cultivars was analyzed, Breeding, identification of seed purity and intellectual property protection of new breeds. The results showed that a total of 35 alleles were detected with 12 pairs of primers. The average number of alleles detected was 1 ~ 6 for each pair of SSR primers, with an average of 2.8. Three pairs of core primers (RM336, RM5414, RM190) were screened The SSR fingerprints of 10 brown planthopper resistant rice plants were established. The similarity coefficients of 10 japonica cultivars ranged from 0.20 to 0.91 with an average of 0.63. The average genetic similarity coefficient of 0.50 was 82.22%. UPGMA clustering results showed that at the genetic similarity coefficient of 0.55, 10 japonica rice could be divided into two groups. The results showed that most of the japonica rice varieties resistant to BPH in Taihu Lake had high genetic similarity and not enough genetic diversity.