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菊花斑枯病为华南地区菊花发生最普遍而严重的一个病害。病原菌鉴定为Septoriachrysanthemella Sacc.,与国内大多数地区所鉴定的相一致,但其生长适温比国外所报道的为高,而与我国台湾省所报道的极为相似,这可能是不同于国外所报道的另一菌系。 此菌在人工培养基上生长极缓慢,形成黑褐色紧密状菌落。合适培养基为燕麦培养基、PDA培养基和菊花叶汁培养基,尤以燕麦培养基为最佳,这是前人所未曾报道的,而文献认为适于Septoria生长的蔗糖硫酸铵等三种人工合成培养基却不适于此菌生长。 此菌产孢适温为20～24℃,在此温度下和合适培养基上产孢所需时间均为16天;光照或黑暗对产孢无影响;产孢量亦以燕麦培养基为最佳。分生孢子萌发适温为26～28℃,适宜pH为5～7,并需要充足水湿、氧气和一定养料,即使在饱和湿度下孢子也不萌发,在嫌气条件下或在清水中孢子不萌发或萌发率极低;在供试的9种糖液中,以鼠李糖(Rhamnose)、葡萄糖(Glucose)、淀粉(starch)、乳糖(Lactose)对孢子萌发较好,最差的为麦芽糖 (Maltose)。
Chrysanthemum spot blight is one of the most common and serious diseases of chrysanthemum in southern China. The pathogen was identified as Septoriachrysanthemella Sacc., Which is consistent with the identification in most of China. However, its growth temperature is higher than that reported in other countries and closely resembles that reported by Taiwan Province of China. This may be different from that reported in foreign countries Another strain of bacteria. The bacteria grew very slowly on artificial media to form dark brown, compact colonies. Suitable medium for oat, PDA and chrysanthemum leaf juice medium, especially the best oat medium, which is previously reported, and the literature that is suitable for the growth of Septoria Sucrose ammonium sulfate and other three Synthetic medium is not suitable for the growth of this bacterium. The sporulation temperature of this strain was 20 ~ 24 ℃. The time required for sporulation at this temperature and appropriate culture medium was 16 days. Light or darkness had no effect on sporulation. The amount of sporulation was also the highest in oat medium good. Conidial germination suitable temperature 26 ~ 28 ℃, suitable pH is 5 ~ 7, and need adequate water wet, oxygen and certain nutrients, even in saturated humidity spores do not germinate, in anaerobic conditions or in water spores No germination or very low germination rate. Among the 9 kinds of sugar solutions tested, germination of spores was better with Rhamnose, Glucose, starch and Lactose, the worst was Maltose.