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目的建立一种新的快速纸层析杂交技术，用于特异性检测带有生物素标记的聚合酶链式反应（ＰＣＲ）扩增产物。方法将特异性捕获探针固定在聚酯砜膜上，扩增产物置膜一端，经毛细作用，带有生物素标记的特异性ＤＮＡ分子固定于反应区，非特异性分子被洗脱，杂交物通过链霉亲和素硷性磷酸酶偶联物及底物（ＢＣＩＰＮＢＴ）显色后判断结果。结果这一技术允许在２５ｍｉｎ内将结核杆菌经ＰＣＲ扩增后的ＤＮＡ１０ｐｇ检出。结论纸层析杂交技术是一种快速、灵敏、简便、价廉、易于推广的ＰＣＲ产物定性检测技术。
Objective To establish a new rapid paper chromatography hybridization technique for the specific detection of polymerase chain reaction (PCR) amplification products with biotinylation. Methods A specific capture probe was immobilized on the polyester sulfone membrane. One end of the membrane was coated with biotin, and the specific DNA molecule with biotin was immobilized on the reaction zone. The non-specific molecules were eluted. The hybrids The results were determined by colorimetric streptavidin-alkaline phosphatase conjugate and substrate (BCIP-NBT). Results This technique allows detection of Mycobacterium tuberculosis by PCR amplification of 10 pg of DNA within 25 min. Conclusion Paper chromatography hybridization is a rapid, sensitive, simple, inexpensive, easy to promote the qualitative detection of PCR products.