论文部分内容阅读
Objective:To elucidate free radical scavenging activity of ethanolic extract Lagenaria siceraria (L.siceraria)(Molina) fruit.Methods:The free radical scavenging activity of the L siceraria (Molina) fruit extract was assayed by usingα,α-diphenyl-β-picrylhydrazyl(DPPH). 2,20-azinobis 3-ethyl henzothiazoline-6-sulfonate(ABTS),FRAP,reducing power,chelating ability andβ-carotene bleaching assay.Results:The IC_(50)values of DPPH and ABTS radicalscavenging activity was found to be 1.95 mg/mL and 19 mg/mL,respectively.In ferrous chelation assay,the percentage of inhibition was found to be 89.21%.The reducing power of ethanolic extract of L siceraria(Molina) fruit was 0.068 at 1 mg/mL and increased to 0.192 at 5 mg/mL Theβ-carotene linoleate bleaching assay was 46.7%at 5 mg/mL and antioxidant activity using FRAP at 0.305 for 1 mg/mL to 0.969 for 5 mg/mL Conclusions:The results indicate that L siceraria (Molina) fruit could be an important sources of natural radical scavengers.
Objective: To elucidate free radical scavenging activity of ethanolic extract Lagenaria siceraria (L.siceraria) (Molina) fruit. Methods: The free radical scavenging activity of the L siceraria (Molina) fruit extract was assayed by using α, α-diphenyl- picrylhydrazyl (DPPH) 2,20-azinobis 3-ethyl henzothiazoline-6-sulfonate (ABTS), FRAP, reducing power, chelating ability and β- carotene bleaching assay. Results: The IC 50 values of DPPH and ABTS radical scavenging activity was found to be 1.95 mg / mL and 19 mg / mL, respectively. In the ferrous chelation assay, the percentage of inhibition was found to be 89.21%. The reducing power of ethanolic extract of L siceraria (Molina) fruit was 0.068 at 1 mg / mL and increased to 0.192 at 5 mg / mL Theβ-carotene linoleate bleaching assay was 46.7% at 5 mg / mL and antioxidant activity using FRAP at 0.305 for 1 mg / mL to 0.969 for 5 mg / mL Conclusions: The results indicate that L siceraria (Molina) fruit could be an important sources of natural radical scavengers.