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目的掌握了解四川省钩端螺旋体基因分型情况、基因型地理分布特征、各地区的主要优势菌型,为分子流行病学调查中确定暴发流行、追溯传染源和传播媒介、预防控制疫情提供科学依据。方法采用多位点可变数目串联重复序列分析方法(MLVA)对四川分离保存的185株钩体菌株7个VNTR位点进行检测,并用BioNumerics软件进行基因分型聚类。结果 185株钩体菌株成功扩增出164株,阳性率为为88.6%,分为81个MLVA基因型,其中156株致病性钩端螺旋体地方株成功扩增出144株,阳性率为92.3%,分为65个MLVA基因型,涵盖11个血清群34个血清型。结论四川地区钩端螺旋体菌多态性好,基因型别与地理分布呈相关性,可通过MLVA方法为钩体疫情的分子流行病学调查提供及时和准确的科学依据。
Objective To understand the genotyping and genotypic distribution of Leptospira in Sichuan Province and the dominant dominant bacteria in different regions in order to provide a scientific basis for identifying epidemic outbreaks, tracing the source of infection and transmission media and preventing and controlling epidemic outbreaks in molecular epidemiological investigation in accordance with. Methods Seven VNTR loci of 185 strains of Leptospira strains isolated and preserved in Sichuan were detected by MLVA with multiple loci and the number of VNTRs was detected by BioNumerics software. Results A total of 185 strains of leptospira were successfully amplified. The positive rate was 88.6%, which was divided into 81 MLVA genotypes. Among them, 144 strains of pathogenic Leptospira were successfully amplified, and the positive rate was 92.3 %, Divided into 65 MLVA genotypes, covering 11 serogroups 34 serotypes. CONCLUSIONS Leptospira bacteria in Sichuan Province have good polymorphism and genotypes are closely related to geographical distribution. The MLVA method can provide timely and accurate scientific evidence for molecular epidemiological investigation of leptospirosis.