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目的:研究川芎嗪对 L-谷氨酸(L-Glu)、5-羟色胺(5-HT)、去甲肾上腺素(NE)等神经递质所致新生鼠脑细胞游离钙([Ca~(2+)]_i)升高的影响。方法:采用血清药理学方法,以 Fura-2/AM 为荧光指示剂,测定脑细胞内[Ca~(2+)]_i 浓度的变化。结果:(1)10%川芎嗪含药血清(TMPS)在含钙及无钙人工脑脊液(ACSF)中均可降低静息脑细胞内[Ca~(2+)]_i 浓度。(2)在含钙1.0 mmol/L 的 ACSF 中,10%TMPS 对150、300、450μmol/L 的 L-Glu 及100、200、300 μmol/L 的5-HT 与0.01、0.1、1.0、10.0 μmol/L 的 NE 所致[Ca~(2+)]_i 浓度升高均有不同程度的抑制作用,同时,TMPS 对 L-Glu、5-HT、NE 等神经递质所致新生鼠脑细胞[Ca~(2+)]_i 升高的抑制作用呈现量效关系。结论:川芎嗪对静息脑细胞[Ca~(2+)]_i 及 L-Glu、5-HT、NE 所致[Ca~(2+)]_i 浓度升高均有抑制作用,这可能是其防治缺血性中风的机理之一。
Objective: To study the effects of tetramethylpyrazine on intracellular free calcium in neonatal rat brain cells induced by neurotransmitters such as L-glutamate (L-Glu), serotonin (5-HT), and norepinephrine (NE) ([Ca~( 2+)]_i) Effect of increase. METHODS: The serum [Ca 2+ ]_i concentration in brain cells was measured using serum pharmacology and Fura-2/AM as fluorescent indicator. Results: (1) 10% Ligustrazine drug-containing serum (TMPS) can reduce the concentration of [Ca 2+ ]_i in resting brain cells in both calcium-containing and calcium-free artificial cerebrospinal fluid (ACSF). (2) In ACSF with 1.0 mmol/L calcium, 10% TMPS vs 150, 300, and 450 μmol/L L-Glu and 100-, 200-, 300 μmol/L 5-HT and 0.01, 0.1, 1.0, 10.0 The increase of [Ca 2+ ]_i concentration induced by μmol/L of NE has different degrees of inhibition. At the same time, TMPS affects neurotransmitters such as L-Glu, 5-HT, NE and other neonatal rat brain cells. The inhibitory effect of [Ca~(2+)]_i elevation showed a dose-effect relationship. Conclusion: Ligustrazine can inhibit the increase of [Ca 2+ ]_i concentration induced by resting brain cells [Ca 2+ ]_i and L-Glu, 5-HT, and NE. This may be One of the mechanisms of its prevention and treatment of ischemic stroke.