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目的探讨核苷酸结合寡聚化结构域(NOD)受体在高脂膳食诱导的不同肥胖易感大鼠骨骼肌胰岛素抵抗中的作用。方法将雄性SD大鼠随机分为对照组和高脂组,分别喂以基础饲料和高脂饲料,8周后高脂组筛选出肥胖易感组和肥胖抵抗组,继续喂养至19周进行口服葡萄糖耐量试验(OGTT),20周后处死大鼠,比较Lee’s指数、脂/体比和胰岛素抵抗指数(HOMA-IR),实时荧光定量PCR检测骨骼肌葡萄糖转运体4(GLUT4)、NOD1和NOD2 mRNA表达水平。结果与对照组和肥胖抵抗组比较,肥胖易感组大鼠体重[(770.1±45.9)g]、脂/体比[(7.1±0.6)%]、空腹胰岛素水平[(74.76±6.49)μIU/mL]、HOMA-IR(3.03±0.10)均明显升高(P<0.01);与对照组比较,肥胖抵抗组大鼠体重无明显变化,Lee’s指数(313.9±7.3)、脂/体比[(5.6±0.8)%]和HOMA-IR(1.77±0.11)明显升高(P均<0.05);与对照组和肥胖抵抗组比较,肥胖易感组大鼠骨骼肌组织GLUT4mRNA(0.66±0.09)表达下调(P均<0.05),NOD2 mRNA(1.27±0.07)表达上调(P<0.05)。结论高脂膳食可能通过激活大鼠骨骼肌NOD2表达参与该组织胰岛素抵抗。
Objective To investigate the role of nucleotide binding oligomerization domain (NOD) receptors in the insulin resistance of skeletal muscle induced by high-fat diet in different obesity susceptible rats. Methods Male Sprague-Dawley rats were randomly divided into control group and hyperlipidemic group, which were fed with basal diet and high-fat diet respectively. After 8 weeks, obese and obesity-resistant group were screened in hyperlipidemia group and continued to be fed for 19 weeks After 20 weeks, the rats were sacrificed and the Lee’s index, lipid / body ratio and insulin resistance index (HOMA-IR) were compared. The expressions of GLUT4, NOD1 and NOD2 were detected by real-time fluorescence quantitative PCR. mRNA expression level. Results Compared with control group and obesity resistance group, body weight, fat / body ratio (7.1 ± 0.6)%, fasting insulin level [(74.76 ± 6.49) μIU / (P <0.01). Compared with the control group, there was no significant change in the body weight of obesity resistance rats (Lee’s index: 313.9 ± 7.3, 5.6 ± 0.8)%] and HOMA-IR (1.77 ± 0.11) (P <0.05). Compared with control group and obesity resistance group, the expression of GLUT4 mRNA in skeletal muscle of obesity susceptible group (0.66 ± 0.09) (All P <0.05), NOD2 mRNA (1.27 ± 0.07) was up-regulated (P <0.05). Conclusion High-fat diet may participate in the insulin resistance through activation of NOD2 in skeletal muscle of rats.