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测定了鳜传染性脾肾坏死病毒 (ISKNV)核糖核酸酶III(RNaseIII)基因的核苷酸全序列。该基因完整读码框为 771bp ,GC含量为 5 2 .5 3% ,编码一个长为 2 5 6个氨基酸、分子量为 2 8.9kD的推定蛋白。其上下游序列各有一个TATAbox ,终止子下游有一段回文序列。与其它物种相比 ,ISKNV与虹彩病毒 (包括LCDV - 1和CIV)的核糖核酸酶III基因的氨基酸序列同源性较高 ,与酵母、线虫等物种的相应基因的同源性较低
The complete nucleotide sequence of the RNaseIII gene of the infectious spleen and kidney necrosis virus (ISKNV) was determined. The complete reading frame of this gene was 771bp with a GC content of 52.33% and encoded a putative protein with a length of 265 amino acids and a molecular weight of 8.9kD. The upstream and downstream sequences have a TATAbox, terminator has a palindromic sequence downstream. Compared with other species, ISKNV has higher amino acid sequence homology with RNase III gene of iridescent virus (including LCDV - 1 and CIV), lower homology with corresponding genes of yeast, nematode and other species