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目的:探讨梓醇对高糖诱导SH-SY5Y神经细胞凋亡的保护作用及其机制。方法:将细胞随机分为对照组、凋亡组、梓醇组、梓醇保护组及甘露醇组。凋亡组用含50 mmol/L葡萄糖的培养液培养SH-SY5Y细胞48 h;梓醇保护组的培养液中分别加入1、2、4 mg/ml梓醇和50 mmol/L葡萄糖,培养48 h。通过MTT比色法检测细胞存活率,Hoechst-33258染色观察细胞核形态变化,流式细胞仪检测细胞凋亡率,酶联免疫吸附法检测细胞上清液中8-羟基脱氧鸟苷酸(8-hydoxydeoxyguanosine,8-OHdG)的含量。结果:与对照组相比,凋亡组细胞存活率明显降低;细胞凋亡率明显升高;Hoechst33258染色细胞核出现凋亡小体;上清中8-OHdG含量明显升高。与凋亡组相比,1,2,4 mg/ml梓醇保护组细胞存活率均明显升高;Hoechst33258染色发现细胞核形态明显改善,细胞核固缩减少;梓醇保护组细胞凋亡率及上清液中8-OHdG的分泌量均明显降低。结论:梓醇对高糖诱导的SH-SY5Y细胞凋亡具有保护作用,其机制可能与梓醇的抗氧化作用有关。
Objective: To investigate the protective effect of catalpol on the apoptosis of SH-SY5Y neurons induced by high glucose and its mechanism. Methods: The cells were randomly divided into control group, apoptosis group, catalpol group, catalpol protective group and mannitol group. SH-SY5Y cells were cultured with 50 mmol / L glucose for 48 h in the apoptotic group; Catalpol-protected groups were treated with 1, 2, 4 mg / ml catalpol and 50 mmol / L glucose respectively for 48 h . The cell viability was detected by MTT colorimetric assay. The morphological changes of nuclei were observed by Hoechst-33258 staining. The apoptosis rate was detected by flow cytometry. The levels of 8-hydroxydeoxyguanosine (8- hydoxydeoxyguanosine, 8-OHdG) content. Results: Compared with the control group, the survival rate of apoptotic cells was significantly decreased; the apoptosis rate was significantly increased; apoptotic bodies were observed in Hoechst33258 staining cells; the content of 8-OHdG in the supernatant was significantly increased. Compared with the apoptotic group, the cell viability of the cells treated with 1, 2, 4 mg / ml catalpol significantly increased. The morphological changes of the cells were observed by Hoechst33258 staining and the nuclear condensation was decreased. Serum 8-OHdG secretion were significantly reduced. Conclusion: Catalpol has a protective effect on the apoptosis of SH-SY5Y cells induced by high glucose. The mechanism may be related to the anti-oxidative effect of catalpol.