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提出并建立了一种用“光子”作试剂代替各种氧化剂的维生素B_1光化学反应——FIA荧光分析法,对反应体系的介质,注样速度,注样管长度以及注样方式进行了实验和讨论,维生素B_1浓度在0～2.0μg/ml范围内呈良好的线性关系,r=0.999,检出限为2.0ng/ml(S/N=3),对9份含维生素B_1为0.10μg/ml的试液进行平行测定,相对标准偏差为2.7％,对维生素B_1片剂分析,测得维生素B_1含量为标示量的94％,标准加入回收率为97％～100％,对维生素B_1注射液分析,测得维生素B_1含量为标示量的100％,标准加入回收率为95％～100％,在尿液基质中做标准加入回收实验,回收率为80％～83％。
A photochemical reaction of vitamin B_1 with “photon” as a reagent instead of various oxidants was proposed and established. FIA fluorescence analysis was used to test the medium, injection speed, injection tube length and injection mode of the reaction system. Discussion, vitamin B_1 concentration in the range of 0 ~ 2.0μg / ml showed a good linear relationship, r = 0.999, the detection limit of 2.0ng / ml (S / N = 3), nine of the vitamin B_1 containing 0.10μg / ml of the test solution for the parallel determination of the relative standard deviation of 2.7%, vitamin B_1 tablet analysis, the measured vitamin B_1 content of 94% of the labeled amount, the standard addition recovery of 97% to 100%, vitamin B_1 injection Analysis, the measured content of vitamin B_1 100% of the labeled amount, the standard addition of 95% to 100% recovery in the urine matrix standards to join the recovery experiment, the recovery rate of 80% to 83%.