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目的:观察中药柴朴汤对哮喘豚鼠支气管肺组织中Bcl-2表达的影响。方法:40只健康豚鼠随机分为正常对照组,哮喘模型组,地塞米松组(0.001 g·kg-1),柴朴汤组(0.35 g·kg-1),每组10只。采用卵蛋白腹腔注射和雾化吸入激发建立哮喘模型,各组豚鼠均于激发前30 min予相应灌胃处理,隔日1次,共7次。末次激发24 h后,处死各组豚鼠。检测各组豚鼠血液中嗜酸性粒细胞数及支气管肺泡灌洗液(BALF)中白细胞(WBC)数,嗜酸粒细胞(EOS)数及百分比;HE染色法观察各组豚鼠支气管肺组织病理改变;免疫组化法检测支气管肺组织中Bcl-2蛋白表达,原位杂交法检测支气管肺组织中Bcl-2 mRNA的表达。结果:柴朴汤组外周血中EOS数,BALF中WBC数、EOS数及百分比均较哮喘模型组明显降低(P<0.05)。与哮喘模型组比较,柴朴汤组支气管肺组织中Bcl-2 mRNA及蛋白的表达有显著性差异(P<0.05)。地塞米松组与柴朴汤组比较,无明显差异。结论:柴朴汤可以明显降低哮喘豚鼠外周血和支气管肺泡灌洗液中EOS,减轻支气管肺组织的炎症反应,下调Bcl-2 mRNA和蛋白的表达水平。
Objective: To observe the effect of Chai Pu soup on the expression of Bcl-2 in bronchopulmonary tissue of asthmatic guinea pigs. Methods: Forty healthy guinea pigs were randomly divided into normal control group, asthma model group, dexamethasone group (0.001 g · kg -1) and Chai Pu Tang group (0.35 g · kg -1), with 10 rats in each group. The model of asthma was established by intraperitoneal injection of nebulizer and inhalation of nebulization. The guinea pigs in each group were treated with gavage 30 min before the challenge, and every other day for 7 times. 24 h after the last challenge, each group of guinea pigs were sacrificed. The number of eosinophils and the number of white blood cells (WBC) and the number of eosinophils (EOS) in BALF of guinea pigs in each group were detected. The pathological changes of bronchopulmonary tissues were observed by HE staining The expression of Bcl-2 protein in bronchial lung tissue was detected by immunohistochemistry. The expression of Bcl-2 mRNA in bronchial lung tissue was detected by in situ hybridization. Results: The number of EOS, the number of WBC in BALF, the number of EOS and the percentage of EOS in Chaopu Decoction group were significantly lower than those in asthma model group (P <0.05). Compared with the asthma model group, the expression of Bcl-2 mRNA and protein in the bronchial lung tissue of Chai Pui Tang group was significantly different (P <0.05). Dexamethasone group and Chai Pu soup group, no significant difference. Conclusion: Chai Pu Tang can significantly reduce the EOS of bronchial and bronchoalveolar lavage fluid of asthmatic guinea pigs, reduce the inflammatory reaction of bronchial lung tissue and downregulate the expression of Bcl-2 mRNA and protein.