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滴灌带中辣椒素含量是确定防鼠型滴灌带质量的重要指标。本文用反相高效液相色谱法对滴灌带中辣椒素的含量进行测定研究。采用甲醇超声提取法提取滴灌带中辣椒素,以1.0mL流速的水/甲醇为流动相,在激发波长229nm,发射波长320nm下进行检测,得出了结论:滴灌带试样的尺寸、滴灌带产品的使用时间、不同标准样对滴灌带产品中辣椒素含量的测定均有影响,且在该色谱条件下,保留时间基本恒定,该方法具有较好的重复性。
The content of capsaicin in drip irrigation is an important index to determine the quality of drip irrigation prevention tape. In this paper, RP-HPLC determination of drip irrigation with capsaicin content. The extraction of capsaicin in the drip irrigation zone was carried out by ultrasonic extraction of methanol, and the mobile phase was 1.0 mL water / methanol. The detection wavelength was 229 nm and the emission wavelength was 320 nm. The conclusion was drawn that the size of drip irrigation zone, The time of using the product and the different standards affect the determination of the capsaicin content in the drip irrigation tape product. Under the chromatographic conditions, the retention time is basically constant, and the method has better repeatability.