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目的探讨凋亡因子Cyt-c、Caspase-9、Caspase-3和凋亡抑制因子Bcl-2、Survivin在宫颈上皮内瘤样病变(cer-vical intraepithelial neoplasia,CIN)及宫颈癌中的表达,以了解它们在宫颈肿瘤发生、发展中的作用。方法采用免疫细胞化学S-P法和图像分析技术检测3种凋亡因子和2种凋亡抑制因子在已确诊的45例宫颈鳞癌、18例腺癌、33例宫颈上皮内瘤样变和23例正常对照的表达情况。结果Cyt-c在腺癌组的表达率明显高于正常组(P<0.01);CIN组及鳞癌组与正常组比较无统计学差异(P>0.05);Cyt-c在腺癌中的表达率高于CIN组和鳞癌组(P<0.05)。Caspase-9在CIN组、鳞癌组、腺癌组的表达阳性率与正常组比较无统计学差异(P>0.05),但腺癌组Caspase-9阳性表达率高于CIN组及鳞癌组(P<0.05)。Caspase-3在腺癌组的阳性表达率高于正常组(P<0.01);在鳞癌组的表达率高于正常组(P<0.05),腺癌组高于CIN组和鳞癌组(P<0.01)。Bcl-2和Survivin在腺癌组、CIN组和鳞癌组的表达率均明显高于正常组(P<0.05),并在腺癌中的表达率最高。结论凋亡因子和凋亡抑制因子的表达可能与宫颈癌的发生、发展密切相关。两者在宫颈癌的发病机制中可能共同起作用。
Objective To investigate the expressions of Cyt-c, Caspase-9, Caspase-3 and Bcl-2 and Survivin in cervical intraepithelial neoplasia (CIN) and cervical carcinoma Understand their role in the development of cervical cancer. Methods Immunocytochemical SP method and image analysis technique were used to detect three kinds of apoptosis factors and two kinds of apoptosis inhibitors in 45 cases of cervical squamous cell carcinoma, 18 cases of adenocarcinoma, 33 cases of cervical intraepithelial neoplasia and 23 cases of cervical intraepithelial neoplasia Normal control expression. Results The expression of Cyt-c in adenocarcinoma was significantly higher than that in normal group (P <0.01). There was no significant difference between CIN and squamous cell carcinoma (P> 0.05) The expression rate was higher than CIN group and squamous cell carcinoma group (P <0.05). The positive rate of Caspase-9 in CIN, squamous cell carcinoma and adenocarcinoma group was not significantly different from that in normal group (P> 0.05), but the positive rate of Caspase-9 in adenocarcinoma was higher than that in CIN and squamous cell carcinoma (P <0.05). The positive rate of Caspase-3 expression in adenocarcinoma group was higher than that in normal group (P <0.01), while in squamous cell carcinoma group was higher than that in normal group (P <0.05) P <0.01). The expression rates of Bcl-2 and Survivin in adenocarcinoma, CIN and squamous cell carcinoma were significantly higher than those in normal group (P <0.05), and were the highest in adenocarcinoma. Conclusions The expressions of apoptosis factor and apoptosis inhibitor may be closely related to the occurrence and development of cervical cancer. Both may play a role in the pathogenesis of cervical cancer.