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目的:探讨镁离子(Mg2+)在0.1mmol/L谷氨酸酸诱发的海马神经元损伤中的作用。方法:将神经元从新生SD大鼠海马中分离后培养6～9d,即单纯随机分成3组。对照组:单纯培养基;谷氨酸组:培养基+5种不同浓度的谷氨酸;镁离子组:培养基+5种不同浓度的Mg2+,30min后再加入0.1mmol/L谷氨酸。结果:①谷氨酸组海马神经元存活率与对照组相比显著地呈剂量依赖性降低,谷氨酸的剂量为2mmol/L时细胞存活率为4.64%。②与谷氨酸组比较,镁离子组低浓度的Mg2+可提高海马神经元的存活率,4.5mmol/LMgSO4处理海马神经元的存活率比谷氨酸组增加78.41%。2+结论:低浓度的Mg能保护谷氨酸诱发损伤的海马神经元。
Objective: To investigate the role of magnesium ion (Mg2 +) in the injury of hippocampal neurons induced by 0.1mmol / L glutamic acid. Methods: Neurons were isolated from the hippocampus of neonatal SD rats and cultured for 6 ~ 9 days. They were randomly divided into 3 groups. Control group: simple culture medium; glutamic acid group: medium +5 different concentrations of glutamic acid; magnesium ion group: medium +5 different concentrations of Mg2 +, 30min and then add 0.1mmol / L glutamic acid. Results: ① The survival rate of hippocampal neurons in glutamate group decreased significantly compared with the control group, and the cell survival rate was 4.64% when glutamate dose was 2mmol / L. ②Compared with glutamate group, low concentration of Mg2 + in magnesium ion group could increase the survival rate of hippocampal neurons. The survival rate of hippocampal neurons increased by 78.41% compared with glutamate group. 2+ Conclusions: Low concentration of Mg protects hippocampal neurons induced by glutamate.