目的 :解决复方卡那霉素眼药水中卡那霉素和地塞米松的含量测定问题。方法 :在复方卡那霉素眼药水中加入衍生试剂 ,使卡那霉素形成二氢吡啶衍生物 ,利用其在紫外有吸收的特点进行测定。地塞米松的测定因受到对羟基苯甲酸乙酯的干扰 ,所以采用双波长分光光度法进行测定。结果 :卡那霉素在 8～ 2 0 μg/ml范围内呈线性r=0 .9998,地塞米松在 1 2～ 2 0 μg/ml范围内呈线性r=0 .9998,卡那霉素和地塞米松的平均回收率分别为 X =99.92 % (RSD为 0 .4 0 % )和 X =99.52 % (RSD为 0 .4 2 % )。结论 :方法快速 ,简便、准确。 Objective: To solve the determination of kanamycin and dexamethasone in compound kanamycin eye drops. Methods: Derivatives were added to kanamycin compound eye drops to make dihydropyridine derivative of kanamycin, which was measured by its absorbability in UV. Dexamethasone was measured by dual wavelength spectrophotometry due to interference from ethylparaben. Results: The linear range of kanamycin in the range of 8 ~ 20 μg / ml was 0.9998. The linear range of dexamethasone in the range of 1 2 ~ 20 μg / ml was r = 0.9998. The kanamycin And dexamethasone were respectively X = 99.92% (RSD 0.40%) and X = 99.52% (RSD 0.42%). Conclusion: The method is rapid, simple and accurate.