LD_(50)值测定原是1927年Trevan为标化剧毒物质如毛地黄、胰岛素和白喉毒素而建立的方法,后来逐渐成为急性毒性的主要观察指标,在药物安全性评价中一直沿用至今.从80年代初到现在,国际毒理学界针对LD_(50)测定召开了多次国际会议,发表了很多文章,一致倾向于反对把经典的LD_(50)测定列为一切新药评价的常规;反对把LD_(50)测定与急性毒性等 The LD_ (50) value was originally established by Trevan in 1927 for the standardization of virulent substances such as foxglobin, insulin and diphtheria toxin, and later gradually became the main observation indicator of acute toxicity and has been used in the evaluation of drug safety until now. From the early 1980s to the present, international toxicology community has held many international conferences and published many articles on LD 50 determination. They are unanimously inclined to oppose the inclusion of the classic LD 50 determination as a general rule in evaluating new drugs. Opposition The LD_ (50) determination and acute toxicity