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为探索农抗702在植物病害生物防治中的实际应用价值及对病原真菌的作用机理,采用生长速率法测定其抑菌谱,并以水稻纹枯病菌为研究对象,测定其作用下菌丝的电解质渗漏、蛋白质、核酸、Mg2+、K+含量的变化,及其对细胞膜麦角甾醇生物合成和水稻纹枯病菌超显微结构的影响.结果表明:农抗702对供试13种病原真菌均有较强的抑制作用,其中对油菜菌核病菌的作用最强,EC50为0.23μg·mL-1;农抗702作用水稻纹枯病菌菌体后相对电导率增加72.2%;蛋白质、核酸、Mg2+、K+等胞内物质均发生了不同程度的渗漏;细胞麦角甾醇含量降低了92.0%;细胞膜轮廓不清、破损;细胞器严重损伤,有空泡化.抑制麦角甾醇合成、造成真菌细胞膜渗透增加是农抗702抑制真菌的主要机理.
In order to explore the practical application value of Nongkang 702 in the biological control of plant diseases and its mechanism of action to pathogenic fungi, the antibacterial spectrum of Nongkang 702 was determined by the growth rate method. Rhizoctonia solani was taken as the research object, The results showed that the effects of Nongkang 702 on the pathogenicity of 13 pathogenic fungi The EC50 was 0.23μg · mL-1. The relative electric conductivity of Rhizoctonia solani increased by 72.2% after the application of Nongkang 702, and the content of protein, nucleic acid , Mg2 +, K + and other intracellular substances have different levels of leakage; ergosterol content decreased 92.0%; cell membrane unclear, damaged; organelles were severely damaged, vacuolar. Inhibition of ergosterol synthesis, resulting in fungal cell membrane Increased osmosis is the main mechanism by which Agrobacterium 702 inhibits fungi.