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目的在体观察失血性休克条件下ＧＲ的变化．以及给予ＧＲ阻断剂ＲＵ４８６后ＰＭＮ粘附的变化，然后采用离体实验。研究ＧＲ阻断后对ＴＮＦ诱导的大鼠ＰＭＮ粘附的作用，为进一步阐明ＧＣ的抗炎机制提供理论依据。方法１．在体实验。（１）ＧＲ测定：测定肝脏的 ＧＲ（Ｓｃａｔｃｈａｒｄ法）和大脑的 ＧＲ（一点法）；（２）ＧＲ阻断后白细胞粘附。观察白细胞贴壁粘附数。２．离体实验。（１）ＰＭＮ与玻璃珠粘附：ＰＭＮ粘附率（％）＝１００％×２．７５×１００％；（２）ＰＭＮ 与 ＥＣ 粘附：ＰＭＮ粘附率（％）＝１００％；（３）ＰＭＮ与ＩＣＡＭ－１包被磁珠粘附测定；（４）ＰＭＮ粘附分子ＣＤ１８检测：ＥＬＩＳＩＡ法。结果１．在体实验。（１）失血性休克组肝细胞胞浆的结合位点与对照组及假手术组相比明显减少，ＧＲ的Ｋｄ值明显增大；脑细胞胞浆ＧＲ的Ｒｓ变化与肝细胞结合位点的变化有相似之处．失血性休克组ＧＲ的Ｒｓ大大低于对照组；（２）ＧＲ阻断＋失血性休克组ＰＭＮ粘附的数目与单纯失血性休克对照组相比ＰＭＮ贴壁粘附数目明显增多；８０％阻断组较之５０％阻断组更加明显。２．离体实验。（１）ＰＭＮ与玻璃珠粘附：随着Ｄｅｘ浓度的增大，ＰＭＮ粘附率逐渐降低（Ｄｅｘ１?
Objective To observe the changes of GR under hemorrhagic shock in vivo. And the change of PMN adhesion after GR blocker RU486 was administered, and then used the in vitro experiment. To study the effect of GR block on TNF-induced PMN adhesion in rats, and to provide a theoretical basis for further clarifying the anti-inflammatory mechanism of GC. Method 1. In-vivo experiment. (1) GR determination: determination of liver GR (Scatchard method) and brain GR (one point method); (2) GR block after leukocyte adhesion. Observed leukocyte adhesion adherent number. 2. In vitro experiments. (1) PMN adhesion to glass beads: PMN adhesion rate (%) = 100% × 2.75 × 100%; (2) PMN adhesion to EC: PMN adhesion rate ) PMN and ICAM-1 coated bead adhesion assay; (4) PMN adhesion molecule CD18 detection: ELISIA method. Results 1. In-vivo experiment. (1) The hematopoietic binding sites of hepatocytes in hemorrhagic shock group were significantly decreased compared with those in sham operation group and sham operation group, and the Kd value of GR increased significantly. The changes of Rs in cytoplasm GR of brain cells correlated with the binding sites of hepatocytes Changes are similar. The Rs of GR in hemorrhagic shock group was significantly lower than that in control group. (2) The number of PMN adhesion in GR + hemorrhagic shock group was significantly higher than that in hemorrhagic shock group Broken group is more obvious than 50% blocked group. 2. In vitro experiments. (1) PMN adherence to glass beads: As the concentration of Dex increases, the adhesion rate of PMN decreases gradually (Dex1?