为筛选对昆虫有高毒力的苏云金芽胞杆菌特异性菌株 ,从韩国不同地区土壤中筛选出两株对鳞翅目和双翅目昆虫具有杀虫活性的苏云金芽胞杆菌菌株 K980 5和 K990 3,它们分属于苏云金芽胞杆菌库斯塔克亚种和鲇泽亚种 ,形成典型的双金字塔型伴胞晶体 .两菌株不但对小菜蛾、甜菜夜蛾具有较高的生物活性 ,而且对双翅目昆虫致倦库蚊有毒 .K980 5含有 cry Aa,cry Ab,cry C,cry D和 cry 基因 ,K990 3含有 cry Aa,cry Ab,cry Ac,cry E和 cry 基因 To screen for Bacillus thuringiensis-specific strains with high virulence to insects, two strains of Bacillus thuringiensis K980 5 and K990 3 with insecticidal activity against Lepidoptera and Diptera were screened from soil in different regions of Korea. They belonged to Bacillus thuringiensis Kustakia and Gracheceae subspecies to form typical double pyramidal companion crystals.These two strains not only had high biological activity to Plutella xylostella and Spodoptera exigua, Insect Culex quinquefasciatus is toxic, K980 5 contains cry Aa, cry Ab, cry C, cry D and cry genes, K990 3 contains cry Aa, cry Ab, cry Ac, cry E and cry genes