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作者对114例B细胞性慢性淋巴细胞白血病(CLL)进行细胞遗传学研究。方法是取周围血淋巴细胞置于RPMI1640、胎牛血清培养基中,加EB病毒、脂多糖、蛋白A或美洲商陆分裂素,培养3天后将所获取的细胞,以G或Q带技术作核型分析。对其中获得足够分裂中期细胞的53例进行遗传学评价。男37、女16例,年龄48～88岁。病程1个月～12年,大部分<5年。未治疗者24例,既往曾用苯丁酸氮芥、强的松、环磷酰胺成放射治疗者29例。结果 53例中21例(40%)呈异常核型,32例(60%)为正常核型。特有的克隆性染色体改变,为额外12号染色体(“+12”)唯一异常者8例,“+12”及其它异常者5例,有其它改变而无“+12”者8例。克隆性染色体改变与病程、治疗情况或白血病
The authors conducted a cytogenetic study of 114 B-cell chronic lymphocytic leukemia (CLL). Method is to take peripheral blood lymphocytes placed in RPMI1640, fetal bovine serum medium, add EB virus, lipopolysaccharide, protein A or mercaptosin, cultured 3 days after the cells obtained by G or Q band technology Karyotype analysis. 53 cases in which sufficient metaphase cells were obtained were genetically evaluated. Male 37, female 16 cases, aged 48 to 88 years old. Duration of 1 month to 12 years, most <5 years. Twenty-four non-treated patients had 29 cases of chlorambucil, prednisone and cyclophosphamide. Results Of the 53 cases, 21 (40%) showed abnormal karyotypes and 32 (60%) had normal karyotypes. Unique clonal chromosome changes were extraneous chromosome 8 (“+12”) only 8 cases of abnormal, “+12” and other abnormalities in 5 cases, other changes without “+12” in 8 cases. Clonal chromosome changes and course of disease, treatment or leukemia