目的　为了解医院感染发病率及病原菌对抗生素药物的耐药情况。方法　对我院 6个科室 5 91例住院病例进行调查。结果　 96例次发生医院感染 ,总发病率为 16.2 % ,医院感染病原菌 72 .9% ,为 G-杆菌 ,依次为铜绿假单胞菌、大肠埃希菌、阴沟杆菌、肺炎克雷伯菌等。 12 .9%为 G+球菌、14 .1%为念珠菌。结论　医院感染病原菌的耐药性明显高于院外菌株。 Objective To understand the incidence of nosocomial infections and the pathogenic bacteria resistant to antibiotics. Methods A total of 5 91 hospitalized cases in 6 departments of our hospital were investigated. Results 96 cases of nosocomial infections, the total incidence rate was 16.2%, 72.9% of hospital-acquired pathogens, as G-bacteria, followed by Pseudomonas aeruginosa, Escherichia coli, cloacae, Klebsiella pneumoniae . 12.9% were G + cocci, 14.1% were Candida. Conclusions The drug resistance of pathogenic bacteria in hospital is obviously higher than that in hospital.